Technical Data
Macrophage Colony Stimulating Factor, Recombinant, Human (M-CSF, CSF-1, Lanimostim, Macrophage colony-stimulating factor 1 precursor, MCSF, M-CSF, MGC31930)
Molecular Biology Storage: -70CShipping: RT
Macrophage colony stimulating factor (M-CSF) is produced by many cell types, including fibroblasts, endothelial cells, monocytes and granulocytes. M-CSF was initially identified on the basis of its ability to stimulate the growth of macrophage/granulocyte containing colonies in soft agar cultures. M-CSF stimulates the development of hemopoietic stem cells into macrophages and supports the growth and differentiation of monocytes.

Recombinant corresponding to 158aa from human Macrophage Colony Stimulating Factor expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50 1.0ng/ml ( 1x10e6units/mg). The biological activity was determined by measuring the dose dependent stimulation of proliferation of mouse M-NSF-60 cells. The optimal concentration should be determined for each specific application.

Recommended Dilutions: Optimal dilutions to be determined by the researcher.

Storage and Stability:
Aliquot to avoid repeated freezing and thawing and freeze at -70C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 12 months.

Molecular Weight:
Source: Recombinant, Human (E. coli)
Purity: ~98% (SDS PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder from 5mM Tris, pH 8.0, with 75mM sodium chloride. Reconstitute with sterile dH2O to 0.1-0.5mg/ml. Do not vortex. Further dilutions should be made in aqueous buffers supplemented with carrier protein, such as 0.1% BSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.