Technical Data
Macrophage Inflammatory Protein 1 alpha, Recombinant, Mouse (MIP-1 alpha, CCL3)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to mouse MIP-1a expressed in E. coli.

Molecular Weight:

Biological Activity:
ED50 range=3.0-10ng/ml, determined by the dose dependent chemotaxis of human THP-1 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.

Endotoxin: ~0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Davatelis, G., P. Tekamp-Olson, S.D. Wolpe, K. Hermsen, C. Luedke, C. Gallegos, D. Coit, J. Merryweather, and A. Cerami (1988) Cloning and characterization of a cDNA for murine macrophage inflammatory protein (MIP), a novel monokine with inflammatory and chemokinetic properties. J. Exp. Med. 167:1939-1944. 2, Widmer, U., Z. Yang, S. van Deventer, K.R. Manogue, B. Sherry, and A. Cerami (1991) Genomic structure of murine macrophage inflammatory protein-1 alpha and conservation of potential regulatory sequences with a human homolog, LD78. J. Immunol. 146:4031-4040. 3. Graham, G.J., M.G. Freshney, D. Donaldson, and I.B. Pragnell (1992) Purification and biochemical characterisation of human and murine stem cell inhibitors (SCI). Growth Factors 7:151-160. 4. Sherry, B., Y. Horii, K.R. Manogue, U. Widmer, and A. Cerami (1992) Macrophage inflammatory proteins 1 and 2: an overview. Cytokines 4:117-130 5. Alam, R., P.A. Forsythe, S. Stafford, M.A. Lett-Brown, and J.A. Grant (1992) Macrophage inflammatory protein-1 alpha activates basophils and mast cells. J. Exp. Med. 176:781-786 6. Lord, B.I., C.M. Heyworth, and L.B. Woolford (1993) Macrophage inflammatory protein: its characteristics, biological properties and role in the regulation of haemopoiesis. Int. J. Hematol. 57:197-206. 7. Mantel, C., Y.J. Kim, S. Cooper, B. Kwon, and H.E. Broxmeyer (1993) Polymerization of murine macrophage inflammatory protein 1 alpha inactivates its myelosuppressive effects in vitro: the active form is a monomer. Proc. Natl. Acad. Sci. USA 90:2232-2236. 8. Buckle, A.M., S. Craig, and L.G. Czaplewski (1997) Assays for macrophage inflammatory proteins. Methods Enzymol. 287:127-148.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.