Technical Data
Macrophage Inflammatory Protein 1 alpha, Recombinant, Mouse (MIP-1 alpha, CCL3)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant mouse MIP-1a is a single, non-glycosylated polypeptide chain containing 69 amino acids and having a molecular weight of 7.82kD. The sequence of the first five N-terminal amino acids was determined to be Ala-Pro-Tyr-Gly-Ala. Contains less than 1% dimers and aggregates.

Biological Activity:
The activity is calculated by the ability to chemoattract Balb3/c splenocytes using a concentration of 110ng/ml.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% (HPLC, FPLC, and reducing and non-reducing SDS-PAGE). Endotoxin level is less than 0.1ng/ug (IEU/ug). Purified by chromatographic techniques.
Concentration: As reported
Form: Supplied as a lyophilized powder. No preservatives added. Reconstitute using sterile deionized water to a concentration 100ug/ml. Further dilutions can be made in other aqueous buffers.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.