Technical Data
Macrophage Inflammatory Protein 1alpha, Recombinant, Rat (MIP-1 alpha, CCL3, LD78 alpha)
Molecular Biology Storage: -20CShipping: RT
Rat macrophage inflammatory protein-1a (MIP-1a) is an 8.0kD protein containing 69 amino acid residues. It plays an important role in the inflammatory response of blood monocytes and tissue macrophages.

Source: E. coli

Form: Lyophilized powder without additives.

Stability: The lyophilized powder, though stable at room temperature, is best stored desiccated below 0C. Reconstituted MIP-1a should be stored in working aliquots at -20C.

Purity: 95% by SDS-PAGE analysis and RP-HPLC analysis.

Endotoxin: 0.1ng/ug

Reconstitution: The lyophilized rat MIP-1a is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to t a concentration of 100ng/ul. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.

Biological Activity: Rat MIP-1a produces chemotaxis over a wide concentration range in an assay using rat peritoneal macrophages. Significant chemotaxis was achieved using a concentration of 200ng/ml.

Concentration Range for most in vitro applications: 100ug/ul

Molecular Weight:
Source: Rat

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.