Technical Data
M1202-10B
Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3)
10ug
|
Technical Data |
|
M1202-10B |
Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
|
Source: Recombinant corresponding to rat MIP-1a expressed in E. coli. Molecular Weight: ~8kD Biological Activity: ED50 range=3.0-10ng/ml, determined by the dose dependent chemotaxis of human THP-1 cells. The optimal concentration for each specific application should be determined by an initial dose response assay. Endotoxin: ~0.1ng/ug Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~8kD |
Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
1. Shanley, T.P., H. Schmal, H.P. Friedl, M.L. Jones, and P.A. Ward (1995) Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats. J. Immunol. 154:4793-4802. 2. Shi, M.M., J.J. Godleski, and J.D. Paulauskis (1995) Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages. Biochem. Biophys. Res. Commun. 211:289-295. 3. Buckle, A.M., S. Craig, and L.G. Czaplewski (1997) Assays for macrophage inflammatory proteins. Methods Enzymol. 287:127-148. 4. : Shi, M.M., I.W. Chong, N.C. Long, J.A. Love, J.J. Godleski, and J.D. Paulauskis (1998) Functional characterization of recombinant rat macrophage inflammatory protein-1 alpha and mRNA expression in pulmonary inflammation. Inflammation 22:29-43. 5. Scheven, B.A., J.S. Milne, I. Hunter, and S.P. Robins (1999) Macrophage inflammatory protein-1 alpha regulates preosteoclast differentiation in vitro. Biochem. Biophys. Res. Commun. 254:773-778. 6. Takano, K., M. Al-Mokdad, F. Shibata, H. Tsuchiya, and H. Nakagawa (1999) Rat macrophage inflammatory protein-1 alpha, a CC chemokine, acts as a neutrophil chemoattractant in vitro and in vivo. Inflammation 23:411-424.
|
||