Technical Data
Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to rat MIP-1a expressed in E. coli.

Molecular Weight:

Biological Activity:
ED50 range=3.0-10ng/ml, determined by the dose dependent chemotaxis of human THP-1 cells. The optimal concentration for each specific application should be determined by an initial dose response assay.

Endotoxin: ~0.1ng/ug

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile ddH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Shanley, T.P., H. Schmal, H.P. Friedl, M.L. Jones, and P.A. Ward (1995) Role of macrophage inflammatory protein-1 alpha (MIP-1 alpha) in acute lung injury in rats. J. Immunol. 154:4793-4802. 2. Shi, M.M., J.J. Godleski, and J.D. Paulauskis (1995) Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar macrophages. Biochem. Biophys. Res. Commun. 211:289-295. 3. Buckle, A.M., S. Craig, and L.G. Czaplewski (1997) Assays for macrophage inflammatory proteins. Methods Enzymol. 287:127-148. 4. : Shi, M.M., I.W. Chong, N.C. Long, J.A. Love, J.J. Godleski, and J.D. Paulauskis (1998) Functional characterization of recombinant rat macrophage inflammatory protein-1 alpha and mRNA expression in pulmonary inflammation. Inflammation 22:29-43. 5. Scheven, B.A., J.S. Milne, I. Hunter, and S.P. Robins (1999) Macrophage inflammatory protein-1 alpha regulates preosteoclast differentiation in vitro. Biochem. Biophys. Res. Commun. 254:773-778. 6. Takano, K., M. Al-Mokdad, F. Shibata, H. Tsuchiya, and H. Nakagawa (1999) Rat macrophage inflammatory protein-1 alpha, a CC chemokine, acts as a neutrophil chemoattractant in vitro and in vivo. Inflammation 23:411-424.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.