Technical Data
Macrophage Inflammatory Protein 1 alpha, Recombinant, Rat (MIP-1a, CCL3)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant Rat MIP-1a produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 69 amino acids and having a molecular mass of 7853 Dalton. Recombinant Rat MIP-1a is purified by proprietary chromatographic techniques.

Amino Acid Sequence:
The sequence of the first five N-terminal amino acids: Ala-Pro-Tyr-Gly-Ala.

Biological Activity:
Recombinant Rat MIP-1a is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract of rat peritoneal macrophages using a conc. of 60 ng/ml.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.38 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of MIP-1 alpha as a Reference Standard.

Reconstitute in ddH2O not less than 100g/ml, which can then be further diluted to other aqueous solutions.

Lyophilized MIP-1alpha although stable at room temperature for 3 weeks, should be stored desiccated below -180C. Upon reconstitution MIP-1 alpha should be stored at 40C between 2-7 days and for future use below -180C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please avoid freeze-thaw cycles.

Molecular Weight:
Source: E. coli
Purity: 95% as determined by RP-HPLC, or SDS-PAGE.
Concentration: 1mg/ml
Form: Supplied as a lyophilized powder without additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. [Expression and significance of mRNA for MIP-1alpha in cerebral tissue of newborn rat with hypoxic-ischemic brain damage]
Sichuan Da Xue Xue Bao Yi Xue Ban 2005 Mar;36(2):240-2
2. Exogenous nitric oxide downregulates MIP-2 and MIP-1alpha chemokines and MAPK p44/42 after ischemia and reperfusion of the rat kidney.
J Invest Surg 2002 Sep-Oct;15(5):287-96
3. Concentration- and time-dependent upregulation and release of the cytokines MIP-2, KC, TNF, and MIP-1alpha in rat alveolar macrophages by fungal spores implicated in airway inflammation.
Am J Respir Cell Mol Biol 1998 Mar;18(3):435-40
4. Induction of macrophage inflammatory protein MIP-1alpha mRNA on glial cells after focal cerebral ischemia in the rat.
Neurosci Lett 1997 May 23;227(3):173-6

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.