Technical Data
M1202-65
Macrophage Inflammatory Protein 3 beta, Recombinant, Human (MIP-3b, Exodus-3, ELC)
5ug
20ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
MIP-3 beta is a CC chemokine that is expressed in the thymus, lymph nodes and in activated bone marrow stromal cells and signals through the CCR7 receptor. MIP-3 beta is a chemoattractant for T and B lymphocytes and myeloid progenitor cells. Human MIP-3 beta is active on murine cells. Recombinant human MIP-3 beta is an 8.8kD protein containing 77 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.

AA Sequence:
GTNDAEDCCL SVTQKPIPGY IVRNFHYLLI KDGCRVPAVV FTTLRGRQLC APPDQPWVER IIQRLQRTSA KMKRRSS

Reconstitution:
Reconstitute with sterile dH2O to 0.1-1.0mg/ml. This solution can then be diluted into other buffers and stored at 4C for 1 week or stored at -20C for future use.

Biological Activity:
Determined by its ability to chemoattract human T cells using a concentration of 10-50ng/ml.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O, aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
8.8kD
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug (1EU/ug)
Concentration: As reported
Form: Supplied as a lyophilized powder from a sterile filtered solution, lyophilized without additives. Reconstitute with sterile dH2O to 0.1-1.0mg/ml. This solution can then be diluted into other buffers.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.