Technical Data
M1202-68D
Macrophage In ammatory Protein 3 alpha, Recombinant, Mouse (MIP-3a, Exodus, LARC)
5ug
20ug
100ug
Growth Factors, Cytokines Storage: -70CShipping: Blue Ice
Recombinant Mouse MIP-3beta produced in E. coli is a single, non-glycosylated, polypeptide chain containing 83 amino acids and having a molecular mass of 9216 Dalton.

Biological Activity: Recombinant MIP-3 beta is fully biologically active when compared to standard. The Activity is calculated by the ability to chemoattract Human mature dendritic cells using a concentration of 10-100ng/ml.

AA Sequence:
GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
9.3kD
Source: Recombinant, murine, E. coli
Purity: 95% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Dendritic cells express CCR7 and migrate in response to CCL19 (MIP-3beta) after exposure to Helicobacter pylori. Microbes Infect 2006 Mar;8(3):841-50
2. Inhibition of generation of cytotoxic T lymphocyte activity by a CCL19/macrophage inflammatory protein (MIP)-3beta antagonist. J Biol Chem 2004 Sep 24;279(39):40276-82
3. Production of macrophage inflammatory protein 3alpha (MIP-3alpha) (CCL20) and MIP-3beta (CCL19) by human peripheral blood neutrophils in response to microbial pathogens. Infect Immun 2003 Jan;71(1):524-6
4. Characterization of mouse CCX-CKR, a receptor for the lymphocyte-attracting chemokines TECK/mCCL25, SLC/mCCL21 and MIP-3beta/mCCL19: comparison to human CCX-CKR. Eur J Immunol 2002 May;32(5):1230-41
5. Expression of CCR-7, MIP-3beta, and Th-1 chemokines in type I IFN-induced monocyte-derived dendritic cells: importance for the rapid acquisition of potent migratory and functional activities. Blood 2001 Nov 15;98(10):3022-9
6. Neutrophils produce biologically active macrophage inflammatory protein-3alpha (MIP-3alpha)/CCL20 and MIP-3beta/CCL19. Eur J Immunol 2001 Jul;31(7):1981-8

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.