Technical Data
M1202-76
Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)
Description:
The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:100; 10ul lables 1x10e6 cells in 100ul. Membrane permeabilization is required for this application.
Immunohistochemistry (Frozen and Paraffin): 1:100-1:200; Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment.
Optimal working dilutions to be determined by researcher.

Recommended Positive Control:
Human spleen

Recommended Negative Control:
I1904-78R: IgG1: Murine Negative Control

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab)2 (HRP) Pab Rb x Mo
I1904-66C: IgG, F(ab)2 X-adsorbed (HRP) Pab Rb x Mo
I1904-66A: IgG, F(ab)2 (FITC) Pab Rb x Mo

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2617Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human monocytes
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human L1 or Calprotectin molecule. Species Crossreactivity: Rhesus and Cynomolgus monkey, baboon, feline, canine, guinea pig, equine, rabbit, rat, goat, porcine, bovine and Fallow deer.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Grosche, A. et al., (2013) Am. J. Veterinary Research 74; 1281-1290. 1. Brandtzaeg, P., et al., J. Clin. Path. 41: 963-970 (1988). 2. Brandtzaeg, P., et al., Histopathol. 21: 191-196 (1992). 3. Flavell, D.J., Jones, D.B. and Wright, D.H., J. Histochem. Cytochem. 35: 1217-1226 (1987). 4. Gutierrez, M., et al., Vet. Immunol. Immunopathol. 71: 321-334 (1999). 5. Ramsay, A.D., et al., Journal of Pathology 164: 321-328 (1991). 6. Christgau, M., et al., Journal of Histochemistry & Cytochemistry 46(12): 1443-1454 (1998). 7. Perez, J., et al., J. Comp. Path. 121: 385-397 (1999). 8. Obert, L., et al., J. Virol. 76(12): 6311-6322 (2002). 9. Malik, N., et al., Circulation 98: 1657-1665 (1998). 10. Bagavant, H., et al., Am. J. Pathol. 160: 141-149 (2002).