		Technical Data
M1202-76	Macrophage (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)

Description:
The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:100; Membrane permeabilization is required for this application.
Immunohistochemistry (Frozen and Paraffin): 1:100-1:200; Prior to staining paraffin sections, product requires protein digestion pre-treatment e.g. trypsin, 0.1% for 10 minutes or antigen retrieval using heat treatment. Frozen sections do not require pretreatment.
Optimal working dilutions to be determined by researcher.

Recommended Positive Control:
Human spleen

Recommended Negative Control:
I1904-78R: IgG1: Murine Negative Control

Recommended Secondary Antibodies:
I1904-65W: IgG, F(ab’)2 (HRP) Pab Rb x Mo
I1904-66C: IgG, F(ab’)2 X-adsorbed (HRP) Pab Rb x Mo
I1904-66A: IgG, F(ab’)2 (FITC) Pab Rb x Mo

Hybridoma:
NS1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	3H2617	Affinity Purified

Size		Storage	Shipping	Source	Host
200ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human monocytes

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human L1 or Calprotectin molecule. Species Crossreactivity: Rhesus and Cynomolgus monkey, baboon, feline, canine, guinea pig, equine, rabbit, rat, goat, porcine and bovine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Brandtzaeg, P., et al., J. Clin. Path. 41: 963-970 (1988). 2. Brandtzaeg, P., et al., Histopathol. 21: 191-196 (1992). 3. Flavell, D.J., Jones, D.B. and Wright, D.H., J. Histochem. Cytochem. 35: 1217-1226 (1987). 4. Gutierrez, M., et al., Vet. Immunol. Immunopathol. 71: 321-334 (1999). 5. Ramsay, A.D., et al., Journal of Pathology 164: 321-328 (1991). 6. Christgau, M., et al., Journal of Histochemistry & Cytochemistry 46(12): 1443-1454 (1998). 7. Perez, J., et al., J. Comp. Path. 121: 385-397 (1999). 8. Obert, L., et al., J. Virol. 76(12): 6311-6322 (2002). 9. Malik, N., et al., Circulation 98: 1657-1665 (1998). 10. Bagavant, H., et al., Am. J. Pathol. 160: 141-149 (2002).