Technical Data
M1202-76C
Macrophage, Low Endotoxin (L1 Protein, Calprotectin, Cystic Fibrosis Antigen)
Description:
The L1 or Calprotectin molecule is an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. The antigen is expressed by granulocytes, monocytes and by tissue macrophages. Variable results have been reported for staining brain macrophages and microglia.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Flow Cytometry: 1:50-1:200; Membrane permeabilization is required.
Immunohistochemistry (Frozen/Paraffin): 1:100-1:200; Paraffin sections require protein digestion pre-treatment (e.g. 0.1% trypsin) for 10 minutes or antigen retrieval using heat treatment prior to staining the paraffin sections. Frozen sections do not require pretreatment.
Optimal dilutions to be determined by the researcher.

Recommended Positive Control:
Human Spleen

Hybridoma:
NS1 myeloma cells with spleen cells from BALB/c mice.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13H2617Affinity Purified
SizeStorageShippingSourceHost
500ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Human monocytes
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid. No stabilizing proteins or preservatives added. Endotoxin: < 0.01EU/ug.
Specificity:
Recognizes the human L1 or Calprotectin molecule, an intracytoplasmic antigen comprised of a 12kD alpha chain and a 14kD beta chain. Species Crossreactivity: equine, porcine, canine, rabbit, baboon, bovine, guinea pig, rat, goat, feline, Cynomolgus monkey, Rhesus Monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Brandtzaeg, P., et al., J. Clin. Path. 41: 963-970 (1988). 2. Brandtzaeg, P., et al., Histopathology 21: 191-196 (1992). 3. Flavell, D.J., et al., J. Histochem. Cytochem. 35: 1217-1226 (1987). 4. Gutierrez, M., et al., Vet. Immunol. Immunopathol. 71: 321-334 (1999). 5. Ramsay, A.D., et al., J. Pathol. 164: 321-328 (1991). 6. Christgau, M., et al., J. Histochem. Cytochem. 46: 1443-1454 (1998). 7. Perez, J., et al., J. Comp. Path. 121: 385-397 (1999). 8. Obert, L., et al., J. Virol. 76: 6311-6322 (2002). 9. Malik, N., et al., Circulation 98: 1657-1665 (1998). 10. Bagavant, H., et al., Am. J. Pathol. 160: 141-149 (2002).