Technical Data
M1202-77
Macrophage
Description:
Macrophages and histiocytes, which are widely distributed throughout the body, play a crucial role in the body defense mechanisms. They are heterogenous in morphology, cytochemistry, function and surface antigens. Immunohistochemical labeling with monoclonal antibodies is one of the most specific and reliable techniques for the identification and localization of these cells. Monoclonal antibodies to macrophage and histiocyte surface or cytoplasmic antigens are important for the immunodiagnosis of both benign and malignant histiocytic lesions. They are useful in the investigation of macrophage differentiation and maturation and in the diagnosis of various inflammatory, reactive and neoplastic disorders of macrophages.

Applications:
Suitable for use in Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:10
Immunohistochemistry (Frozen): 1:100
Optimal dilutions to be determined by the researcher.

Hybridoma:
Derived from hybridization of mouse SP2/0 myeloma cells with spleen cells from Balb/c mice immunized with WAG/Rij lymphnode cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG16A592Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~0.1mg/ml
Immunogen:
WAG/Rij lymphnode cells
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.01% sodium azide, 50% glycerol.
Specificity:
Recognizes rat macrophages and is reactive with all common inbred strains.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.