Technical Data
Monocyte Chemotactic Protein 1, Recombinant, Rat (MCP-1)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to rat Monocyte Chemotactic Protein 1 expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50=1.0-10ng/ml. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~95% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Yoshimura, T., M. Takeya, and K. Takahashi (1991) Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines. Biochem. Biophys. Res. Commun. 174:504-509. 2. Wareen, J.S., M. Jones, and C. Flory (1993) Analysis of monocyte chemoattractant protein 1-mediated lung injury using rat lung organ cultures. Am. J. Pathol. 143:894-906. 3. Zhang Y., C.A. Ernst, and B.J. Rollins (1996) MCP-1: Structure/activity analysis. Methods 10:93-103. 4. Porreca, E., C. Di Febbo, M. Reale, M. Castellani, G. Baccante, R. Barbacane, P. Conti, F. Cuccurullo, and A. Poggi (1997) Monocyte chemotactic protein 1 (MCP-1) is a mitogen for cultured rat vascular smooth muscle cells. J. Vasc. Res. 34:58-65. 5. Yamashiro, S., M. Takeya, J. Kuratsu, Y. Ushio, K. Takahashi, and T. Yoshimura (1998) Intradermal injection of monocyte chemoattractant protein-1 induces emigration and differentiation of blood monocytes in rat skin. Int. Allergy Immunol. 115:15-23. 6. Turler, A., N. Schward, E. Turler, J. Kalff, and A. Bauer (2002) MCP-1 causes leukocyte recruitment and subsequently endotoxemic ileus in rat. Am. J. Physiol. Gastrointest Liver Physiol. 282:145-155. 7. Czermak, B.J., V. Sarma, N.M. Bless, H. Schmal, H.P. Freidl, and P.A. Ward (1999) In vitro and in vivo dependency of chemokine generation on C5a and TNF-a. J. Immunol. 162:2321-2325.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.