Technical Data
M1203-02R
Monocyte Chemotactic Protein 1, Recombinant, Rat (MCP-1)
10ug
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Technical Data |
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M1203-02R |
Monocyte Chemotactic Protein 1, Recombinant, Rat (MCP-1) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Source: Recombinant corresponding to rat Monocyte Chemotactic Protein 1 expressed in E. coli. Molecular Weight: ~14kD Endotoxin: ~0.1ng/ug Biological Activity: ED50=1.0-10ng/ml. The biological activity was determined by measuring the dose dependent chemotaxis with human THP-1 cells. The optimal concentration should be determined for each specific application by an initial dose-response assay. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~14kD |
Purity: ~95% (SDS-PAGE) Concentration: Not determined Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Yoshimura, T., M. Takeya, and K. Takahashi (1991) Molecular cloning of rat monocyte chemoattractant protein-1 (MCP-1) and its expression in rat spleen cells and tumor cell lines. Biochem. Biophys. Res. Commun. 174:504-509. 2. Wareen, J.S., M. Jones, and C. Flory (1993) Analysis of monocyte chemoattractant protein 1-mediated lung injury using rat lung organ cultures. Am. J. Pathol. 143:894-906. 3. Zhang Y., C.A. Ernst, and B.J. Rollins (1996) MCP-1: Structure/activity analysis. Methods 10:93-103. 4. Porreca, E., C. Di Febbo, M. Reale, M. Castellani, G. Baccante, R. Barbacane, P. Conti, F. Cuccurullo, and A. Poggi (1997) Monocyte chemotactic protein 1 (MCP-1) is a mitogen for cultured rat vascular smooth muscle cells. J. Vasc. Res. 34:58-65. 5. Yamashiro, S., M. Takeya, J. Kuratsu, Y. Ushio, K. Takahashi, and T. Yoshimura (1998) Intradermal injection of monocyte chemoattractant protein-1 induces emigration and differentiation of blood monocytes in rat skin. Int. Allergy Immunol. 115:15-23. 6. Turler, A., N. Schward, E. Turler, J. Kalff, and A. Bauer (2002) MCP-1 causes leukocyte recruitment and subsequently endotoxemic ileus in rat. Am. J. Physiol. Gastrointest Liver Physiol. 282:145-155. 7. Czermak, B.J., V. Sarma, N.M. Bless, H. Schmal, H.P. Freidl, and P.A. Ward (1999) In vitro and in vivo dependency of chemokine generation on C5a and TNF-a. J. Immunol. 162:2321-2325.
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