Technical Data
Macrophage, Monocyte Chemotactic Protein 3, Recombinant, Mouse (MCP-3, MARC)
Molecular Biology Storage: -20CShipping: RT
Recombinant Murine MCP-3 is a non-glycosylated, Polypeptide chain containing 74 amino acids and having a molecular mass of 8510 Dalton.

The sequence of the first five N-terminal amino acids was determined and was found to be Gln-Pro-Ala-Gly-Pro.

Dimers and Aggregates:
1% as determined by silver-stained SDS-PAGE gel analysis.

Biological Activity:
Murine MCP-3 is fully biologically active when compared to standard. The specific activity as determined by the ability of Murine MCP-3 to chemoattract Balb/C mouse spleen MNCs at 10-100ng/ml.

0.1ng/ug (IEU/ug) of Murine Monocyte Chemotactic Protein-3.

Protein Content:
Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280nm.
2. Analysis by RP-HPLC, using a calibrated solution of Murine MCP-3 as a Reference Standard.

Reconstitute the lyophilized MCP-3 in sterile 18MO-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile dH2O and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 98% by RP-HPLC, FPLC, or reducing/non-reducing SDS-PAGE Silver Stain. Chromatographically purified.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder. No additives.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Th2- and to a lesser extent Th1-type cytokines upregulate the production of both CXC (IL-8 and gro-alpha) and CC (RANTES, eotaxin, eotaxin-2, MCP-3 and MCP-4) chemokines in human airway epithelial cells. Meyer-Hoffert U, Lezcano-Meza D, Montes-Vizuet AR, Int Arch Allergy Immunol 2003 Aug;131(4):264-71 2. [Active immunity for anti-colorectal cancer induced by chemokine MCP-3 gene transfection] Hu JY, Li GC, Wang WM, Ai Zheng 2002 May;21(5):504-8 3. Transfection of colorectal cancer cells with chemokine MCP-3 (monocyte chemotactic protein-3) gene retards tumor growth and inhibits tumor metastasis. Hu JY, Li GC, Zhu JG, World J Gastroenterol 2002 Dec;8(6):1067-72 4. Gelatinase B, PECAM-1 and MCP-3 gene polymorphisms in Belgian multiple sclerosis. Nelissen I, Dubois B, Ronsse I, J Neurol Sci 2002 Aug 15;200(1-2):43-8 5. Eotaxin but not MCP-3 induces eosinophil influx into nasal fluid in allergic patients. Gorski P, Wittczak T, Palczynski C, Allergy 2002 Jun;57(6):519-28 6. Transduction of human MCP-3 by a parvoviral vector induces leukocyte infiltration and reduces growth of human cervical carcinoma cell xenografts. Wetzel K, Menten P, Van Damme J, Sozzani S, J Gene Med 2001 Jul-Aug;3(4):326-37.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.