Technical Data
M1203-04H
Macrophage, Monocyte Chemotactic Protein-1, aa24-99, Recombinant, Human (MCP-1, Chemokine C-C Motif Ligand 2, CCL2, GDCF-2, HC11, HSMCR30, JE, MGC9434, Monocyte Chemoattractant Protein 1, Monocyte Chemotactic and Activating Factor, Monocyte Chemotactic Pr
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Chemokine (C-C motif) ligand 2 (CCL2) is a small cytokine belonging to the CC chemokine family that is also known as monocyte chemotactic protein-1 (MCP-1). CCL2 recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4. Recombinant human CCL2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Endotoxin Level:
1.0 EU per 1 ug of protein (determined by LAL method)

AA Sequence:
MGSSHHHHHH SSGLVPRGSH MQPDAINAPV TCCYNFTNRK ISVQRLASYR RITSSKCPKE AVIFKTIVAK EICADPKQKW VQDSMDHLDK QTQTPKT

Molecular Weight: 10.9kD (97 aa)

Storage and Stability:
For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
10.9kD
Source: E. coli
Purity: 95 % by SDS-PAGE
Concentration: ~0.5mg/ml
Form: Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, 1mM DTT, 20% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Serbina NV., et al. (2008) Annu Rev Immunol. 26:421-52 2. Rafei M., et al. (2009) J Immunol. 182(10):5994-6002

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.