Technical Data
Macrophage Scavenger Receptor Type I (MSR1)
Macrophage scavenger receptors (MSR) are membrane glycoproteins that mediate the recognition and uptake of a wide variety of negatively charged macromolecules. There are two types of these receptors that differ in the C-terminal region. MSR has a key role in scavenging a variety of macromolecules, including modified lipoproteins, advanced glycation end (AGEs) products and amyloid beta-protein (Abeta). While the normal role of MSR is associated with cell adhesion and host defense mechanisms, it also has been implicated in the development of atherosclerosis and Abeta deposition in Alzheimer’s disease (AD). The observation that the 4 allele of the apolipoprotein E gene is associated with an increased risk for AD has lead to the investigation of lipoprotein metabolism in the brain of AD patients. The type I MSR has been found to be expressed on microglia and Mato cells, but not astrocytes or neurons. In AD brain, MSR type I is strongly expressed by microglia cells that are associated with senile plaques. Because the senile plaques contain AGEs, which are a source of free radicals that could result in oxidative damage to the brain, MSR may have a role in mediating the endocytosis of AGEs.

Suitable for use in Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:200 (Paraffin embedded, formalin fixed human brain). Immunolabels amyloid plaques in formalin-fixed paraffin sections from Alzheimer’s Disease (AD) brain.
ELISA: 1:100,000 (against immunogen peptide).
Western Blot: 1:2,000

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
200ul4°C (-20°C Glycerol)Blue IceHumanGoat
Not determined
Synthetic peptide that corresponds to aa325-342 of the C-terminus of the human MSR type I.
Supplied as a liquid with 0.01% thimerosal.
Specific for human macrophage scavenger receptor type I (MSR type I).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.