Technical Data
M1250-30
Macrophages, Neutrophils
Description:
Recommended Secondary Reagents: F(ab')2 rabbit anti-rat IgG HRP conjugate

Fusion Partners: Spleen cells from immunized DA rats were fused with cells of the Y3 Ag1.2.3 rat myeloma cell line.

Recommended Negative Controls: Rat IgG2b Negative Control

Applications:
Immunohistochemistry: (frozen)- 1:20
Optimal working dilutions to be determined by researcher.
TypeIsotypeCloneGrade
MabIgG2b3H2625Purified
SizeStorageShippingSourceHost
200Tests-20CBlue IceHumanRat
Concentration:
~200 Tests/500ul
Immunogen:
Human peripheral blood mononuclear cells.
Purity:
Purified by ammonium sulfate precipitation.
Form
Ig fraction - liquid in PBS with 0.1% sodium azide.
Specificity:
Recognizes all tissue macrophages, binding to a cytoplasmic epitope. In peripheral blood the antibody stains all granulocytes and around 50% of monocytes. No staining of osteoclasts or Langerhan's cells is seen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aqel, N.M. et al. (1987). Identification of cells of the human mononuclear phagocyte system with rat monoclonal antibodies. J. Pathol. 52: 265-273.2. Aqel, N.M. et al. (1985). Identification of macrophages and smooth musc