Recommended Secondary Reagents: F(ab')2 rabbit anti-rat IgG HRP conjugate
Fusion Partners: Spleen cells from immunized DA rats were fused with cells of the Y3 Ag1.2.3 rat myeloma cell line.
Recommended Negative Controls: Rat IgG2b Negative Control
Immunohistochemistry: (frozen)- 1:20
Optimal working dilutions to be determined by researcher.
| ~200 Tests/500ul|
|Human peripheral blood mononuclear cells.|
|Purified by ammonium sulfate precipitation.|
|Ig fraction - liquid in PBS with 0.1% sodium azide.|
|Recognizes all tissue macrophages, binding to a cytoplasmic epitope. In peripheral blood the antibody stains all granulocytes and around 50% of monocytes. No staining of osteoclasts or Langerhan's cells is seen.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Aqel, N.M. et al. (1987). Identification of cells of the human mononuclear phagocyte system with rat monoclonal antibodies. J. Pathol. 52: 265-273.2. Aqel, N.M. et al. (1985). Identification of macrophages and smooth musc|