Technical Data
Macrophages, Late Stage Inflammatory
The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. This clone is very useful for macrophage phenotyping.

Can be used for Western Blot, Flow Cytometry, Immunohistochemistry

Recommended Dilution:
Immunohistochemistry: 1:10 (frozen and paraffin sections)
Flow Cytometry: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
MabIgG16A596Affinity Purified
100ug-20CBlue IceHumanMouse
Purified by Protein G affinity chromatography.
Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.
Recognizes a protein of 86kD on the cell surface and within the cytoplasm of mature macrophages. Crossreacts with a subpopulation of macrophages of rhesus monkey, porcine alveolar macrophages and Kupffer cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rosseau, S et al; Phenotypic characterization of alveolar monocyte recruitment in acute respiratory distress syndrome. Am J Physiol Lung Cell Mol Physiol 2000, 279: L25. 2. Zwadlo, G et al; A monoclonal antibody to a differentiation antigen present on human macrophages and absent from monocytes. J Immunol 1985, 134: 1487. 3. Kiefer, R et al; Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies. Brain 1998, 121: 469. 4. Zwadlo, G et al; A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol 1987, 55: 295.