		Technical Data
M1251-01	Macrophages, Late Stage Inflammatory

Description:
The antibody is associated with fully differentiated tissue macrophages both in normal and in diseased tissues, independently of the presence or absence of inflammation. The antigen is absent on freshly isolated monocytes and other blood cells. After 6 to 7 days culture human monocytes become positive. Some melanoma and carcinoma cell lines are also positive. This clone is very useful for macrophage phenotyping.

Applications:
Can be used for Western Blot, Flow Cytometry, Immunohistochemistry

Recommended Dilution:
Immunohistochemistry: 1:10 (frozen and paraffin sections)
Flow Cytometry: 1:10
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG1	6A596	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.1mg/ml

Immunogen:

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, 0.02% sodium azide, 0.1% BSA.

Specificity:

Recognizes a protein of 86kD on the cell surface and within the cytoplasm of mature macrophages. Crossreacts with a subpopulation of macrophages of rhesus monkey, porcine alveolar macrophages and Kupffer cells.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Rosseau, S et al; Phenotypic characterization of alveolar monocyte recruitment in acute respiratory distress syndrome. Am J Physiol Lung Cell Mol Physiol 2000, 279: L25. 2. Zwadlo, G et al; A monoclonal antibody to a differentiation antigen present on human macrophages and absent from monocytes. J Immunol 1985, 134: 1487. 3. Kiefer, R et al; Macrophage differentiation antigens in acute and chronic autoimmune polyneuropathies. Brain 1998, 121: 469. 4. Zwadlo, G et al; A monoclonal antibody to a novel differentiation antigen on human macrophages associated with the down-regulatory phase of the inflammatory process. Exp Cell Biol 1987, 55: 295.