Technical Data
M2141-02
Malondialdehyde (MDA, 1,1,3,3-Tetramethoxypropane)
Description:
MDA (Malondialdehyde) is a highly reactive three carbon dialdehyde produced as a byproduct of polyunsaturated fatty acid peroxidation and arachidonic acid metabolism. MDA readily combines with several functional groups on molecules including proteins, lipoproteins, and DNA. MDA-modified proteins may show altered physico-chemical behavior and antigenicity. Antibodies to MDA will help to visualize the MDA-adducts.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
ELISA: 1:1000-1:10,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20CBlue IceGoat
Concentration:
As reported
Immunogen:
MDA coupled with KLH
Purity:
Serum
Form
Supplied as a lyophilized powder from 0.05% sodium azide. Reconstitute with 100ul sterile dH2O.
Specificity:
Recognizes Malondialdehyde (MDA)-protein adducts in all species
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yahya, ND et al (1996) J. Autoimmunity 9, 3-9; 2. Lung, CC et al (1992) Life Sci. 52, 329-337; 3. Lung et al (1990) J. Immunological Methods 128, 127-132; 4. Shah, G (1994) Life Sci. 55, 1343-1349