Technical Data
M2155-06
Maltose Binding Protein (MBP)
Description:
Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Protein tagging has become a widely applied method for ready purification of proteins. The specific interaction between the tag and a solid-phase ligand allows for the selective binding and elution of the tagged protein. Many vectors that incorporate these tags have been engineered with a cleavage site between the tag and the protein of interest. This permits the separation of the protein from the tag, if needed. One commonly used system is the maltose-binding protein (MBP). In this system, the target gene is inserted in the appropriate vector and expressed. Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein.

Applications:
Suitable for use in ELISA, Immunofluorescence and Western Blot. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2 hours at RT.
Optimal dilutions to be determined by the researcher.

Positive Control:
MBP fusion protein in bacteria, bacterial lysate, or cells and tissues transfected with MBP fusion protein expressing vectors.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.392Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouse
Concentration:
~0.2mg/ml
Immunogen:
Purified recombinant MBP fusion protein. Cellular Localization: Depends upon the localization of the parent protein tagged with MBP.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See M2155-06X.
Specificity:
Recognizes free maltose binding protein (MBP) or MBP fusion proteins at ~53kD. Species Crossreactivity: Bacteria and proteins from all species which are tagged with MBP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.