Technical Data
M2155-07D
Maltose Binding Protein (MBP)
Description:
Recombinant DNA technology allows the addition of short pieces of well-defined tags, "peptides" or proteins at the amino or c-terminus of target genes, which can provide 'affinity handles' designed to bind specific matrices. Therefore, tags enables a selective identification and purification of the protein of interest. The addition of a maltose binding protein (MBP) tag creates a stable fusion product that does not appear to interfere with the activity of the protein or with the cellular localization of the MBP-tagged product (1, 2). The expression of polypeptides in-frame with maltose binding protein (MBP) allows for their easy, single-step purification from bacterial extracts under mild conditions using amylose resin (2). This system utilize a specific protease digestion site to facilitate correct cleavage of the fusion protein (1). Thus, the MBP system incorporates a factor Xa cleavage site at the carboxy terminus of the MBP sequence (3) and cleavage by factor Xa separates MBP from its fusion protein. Many recombinant proteins have been engineered with MBP tags to facilitate the detection, isolation and purification of these proteins (1-6). Ant-MBP may be used in various immunoassay to identify the expression of a MBP fusion protein.

Applications:
Suitable for use in ELISA, Western Blotting. Other applications not tested.

Recommended Dilution:
Western Blotting (1:1000-1:5000 using ECL. Antibodies react with native and denatured his-tag containing proteins.
ELISA: 1:10-50K using 50-100ng control antigen/well.

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13H33Ascites
SizeStorageShippingSourceHost
100ul-20CBlue IceMouse
Concentration:
As reported
Immunogen:
Purified MBP
Purity:
Ascites
Form
Neat ascites in 0.1% sodium azide, 40% glycerol.
Specificity:
Recognizes native and denatured-reduced forms of MBP-fusion proteins in immunoblotting, Dot Blot and ELISA
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Guan, C., et al., Gene, 67, 21 (1988);
2. Maina, C., et al., Gene, 74, 365 (1988).
3. Rodriguez, P., and Carrasco, L., Biotechniques, 18, 238 (1995).
4. Narayanan, S., J. Chromatogr., 658, 237 (1994).
5. Olins, P., and Lee, S., Curr. Opin. Biotechnol. 4, 520 (1993).
6. Uhlen, M., and Moks, T., Meth. Enzymol., 185, 129 (1990).