Technical Data
Maltose Binding Protein (MBP)
Maltose binding protein is a bacterial protein, which is often used in protein expression studies because it creates a stable fusion product that does not appear to interfere with the bioactivity of the protein of interest. It also allows for its easy purification from bacterial extracts under mild conditions. Protein tagging has become a widely applied method for ready purification of proteins. The specific interaction between the tag and a solid-phase ligand allows for the selective binding and elution of the tagged protein. Many vectors that incorporate these tags have been engineered with a cleavage site between the tag and the protein of interest. This permits the separation of the protein from the tag, if needed. One commonly used system is the maltose-binding protein (MBP) (1). In this system, the target gene is inserted in the appropriate vector and expressed. Following purification using an amylose column, the MBP-target protein complex can be eluted from the column with maltose and cleaved with a specific protease to yield the purified target protein (2).

Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:200
Immunocytochemistry (for IF): 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul4C (-20C Glycerol)Blue IceMouse
Not determined.
MBP-fusion protein.
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol, 0.02% sodium azide.
Detects MBP-fusion recombinant proteins. Only recognizes maltose-binding protein. Species Crossreactivity: A broad reactivity is expected due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. di Guan, C. et al. (1988) Gene 67, 2130. 2. Kellerman, O.K. and Ferenci, T. (1982) Methods in Enzymol. 90, 459463.