Technical Data
M2155-09
Maltose Binding Protein
Description:
Through the use of standard molecular biology techniques, a cDNA encoding a protein of interest can be cloned, in-frame, into an expression vector containing the tag sequence. The resultant tagged protein is usually referred to as an epitopetagged protein or a fusion protein. Currently, a variety of expression vectors containing tags are commercially available. Depending on the expression vector selected, these fusion proteins may be expressed in a variety of organisms including bacteria, yeast, insect cells, and mammalian cells. Detection of a tagged protein is facilitated by the use of an antibody directed against the particular tag. This alleviates the need to generate a specific antibody against the protein itself. Therefore, newly identified proteins can be expressed and studied without having to wait for a specific antibody to be generated. Some of the more commonly used fusion tags include: c-Myc, FLAG, GFP, GST, HA, His, and MBP. Maltose binding protein (MBP) vectors are designed for the expression and purification of MBP fusion proteins(13). Generation of the MBP fusion protein is accomplished by fusing a gene of interest to the malE gene of E. coli (2). MBP vectors contain an inducible tac promoter and the malE translation initiation signals which results in high level expression of the resultant MBP fusion protein(1,3). Such fusion proteins bind with high affinity to columns composed of an amylose resin and can be eluted from the column using free maltose. MBP vectors also possess a sequence encoding the recognition site for the protease, factor Xa(1,3). The protease site is positioned in such a manner that factor Xa cleavage can be utilized to separate the protein of interest from MBP.

Applications:
Can be utilized for the detection and purification of MBP-fusion proteins expressed in E. coli. and other hosts. Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 0.11ug/ml
Western Blotting: 12ug/ml
Immunoprecipitation: 5ug/IP reaction

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1,k3G178Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
0.5mg/ml
Immunogen:
Recombinant Maltose Binding Protein derived from the malE gene of E. coli.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.1% sodium azide.
Specificity:
Specific for the detection of maltose binding protein (MBP).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Maina, C.V., et al. (1988) Gene 74:365373. 2. Guan, C. et al. (1987) Gene 67:2130. 3. Riggs, P., (1990) in Current Protocols in Molecular Biology, Ausebel, F.M. et al. (eds.), Greene Publishing Assoc. and Wiley-Interscience, New York.