Technical Data

Media

Mammary Epithelial Basal Medium MCDB 170 (Powder)

M2162

Cell Culture Grade

Storage RT/4°C Shipping RT

Components shown as mg/Liter

Inorganic Salts:
Ammonium Metavandate	0.000585
Ammonium Molybdate•4H2O	0.001236
Calcium Chloride	222.00
Cupric Sulfate	0.000160
Ferrous Sulfate•7H2O	1.39
Magnesium Sulfate	180.57
Manganese Sulfate	0.000121
Nickel Chloride•6H2O	0.000001189
Potassium Chloride	186.40
Potassium Phosphate Monob	68.05
Sodium Chloride	7014.00
Sodium Metasilicate•9H2O	0.1421
Sodium Selenite	0.005188
Stannous Chloride•2H2O	0.000001128
Zinc Sulfate•7H2O	0.0897736

Amino Acids:
L-Alanine	8.909
L-Arginine	52.27
L-Asparagine	132.10
L-Aspartic Acid	13.31
L-Cysteine•HCl	11.029
L-Glutamic Acid	14.71
L-Glutamine	292.20
Glycine	7.507
L-Histidine	15.52
L-Isoleucine	13.12
L-Leucine	39.36
L-Lysine•HCl	36.54
L-Methionine	4.476
L-Phenylalanine	4.956
L-Proline	5.755
L-Serine	31.53
L-Threonine	35.73
L-Tryptophan	6.126
L-Tyrosine	9.06
L-Valine	35.16

Vitamins:
Biotin	0.007329
Choline Chloride	13.96
Vitamin B12	0.13550
Folinic Acid, Calcium	0.005115
Myo-Inositol	18.02
Nicotinamide	6.105
D-Pantothenic Acid•Ca	0.2383
Pyridoxine•HCl	0.06168
Riboflavin	0.11292
Thiamine•HCl	0.3373
Other Components:
Adenine	0.1351
Thymidine	0.07266
D-Glucose	1441.3
HEPES Buffer	7149.00
Lipoic Acid	0.002063
Phenol Red, Sodium	1.242
Putrescine•2HCl	0.000161
Sodium Pyruvate	110.00

Total	17.18g/liter

Basal nutrient medium used for the growth of mammary epithelial cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).

Suggested Supplements: BPE 0.4%, Epidermal Growth Factor 10ng/ml, Insulin 5ug/ml, Hydrocortisone 0.5ug/ml, Isoproterenol 10uM, Transferrin 5ug/ml, Ethanolamine 0.1mM, Phosphoethanolamine 0.1mM, Amphotericin B 50ng/ml,
Gentamicin 50ug/ml

Directions per Liter: Dissolve 17.18g in 800-900ml of ddH2O stirring gently until completely solubilized. Do NOT heat. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to pale orange, homogenous, free flowing powder

Solubility: Light yellow, clear, complete

pH: 5.5±0.4

Endotoxin: 1EU/ml

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Partanen, J.I. et al., (2007) PNAS 104:14694-14699. 1. Hammond, S. L. et al. Proc. Natl. Acad. Sci. USA Vol. 81; 5435-5439 (1984). 2. Peehl, D.M. and Ham, R.G., In Vitro: 16:526 (1980). 3. Bettger, W.J. et al., Proc. Natl, Acad. Soc., USA:78:, 5588-5592 (1981). 4. Stampfer, M.R. and Bartley, J. C. PNAS 82; 2394-2398 (1985)