Technical Data
Media
Mammary Epithelial Basal Medium MCDB 170, w/o HEPES (Powder)
M2162-05
Cell Culture Grade
Storage RT/4°C    Shipping RT
Inorganic Salts: mg/Liter
Ammonium Metavandate0.000585
Ammonium Molybdate•4H2O0.0012359
Calcium Chloride•2H2O222.00
Cupric Sulfate•5H2O0.0002497
Ferric Nitrate•9H2O1.39
Magnesium Sulfate180.57
Manganese Sulfate•5H2O0.0001205
Nickel Chloride•6H2O0.0000011885
Potassium Chloride186.25
Potassium Phosphate Mono68.05
Sodium Chloride7008.00
Sodium Metasilicate•9H2O0.1421
Sodium Selenite•5H2O0.00789
Stannous Chloride•2H2O0.000001128
Zinc Sulfate•7H2O0.14375
Amino Acids:
L-Alanine8.90
L-Arginine52.26
L-Asparagine132.10
L-Aspartic Acid13.31
L-Cysteine8.55
L-Glutamic Acid14.71
L-Glutamine292.00
Glycine7.50
L-Histidine15.52
L-Isoleucine13.12
L-Leucine39.36
L-Lysine29.24
L-Methionine4.48
L-Phenylalanine4.96
L-Proline5.76
L-Serine31.53
L-Threonine35.73
L-Tryptophan6.126
L-Tyrosine9.06
L-Valine35.16
Vitamins:
Biotin0.007329
D-Pantothenic Acid•Ca0.2383
Choline Chloride13.96
Vitamin B120.13554
Folinic Acid,Calcium0.006016
Myo-Inositol18.02
Nicotinamide6.105
Pyridoxine•HCl0.02056
Riboflavin0.11292
Thiamine•HCl0.3373
Other Components:
Adenine0.1351
Thymidine0.07266
D-Glucose1441.6
HEPES Free Acid-
Lipoic Acid0.002063
Phenol Red, Sodium1.242
Putrescine•2HCl0.0001611
Sodium Pyruvate110.00
Total10.02g/liter
Basal nutrient medium used for the growth of mammary epithelial cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).


Directions per Liter: Dissolve 10.02g in 800-900ml of ddH2O stirring gently until completely solubilized. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to pale yellow, homogenous, free flowing powder Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. Peehl, D.M. and Ham, R.G., In Vitro: 16:526 (1980). 2. Bettger, W.J. et al., Proc. Natl, Acad. Soc., USA:78:9, 5588-5592 (1981)