Technical Data
Media
Mammary Epithelial Basal Medium MCDB 170 w/o Calcium, Phenol Red (Powder)
M2162-07
Cell Culture Grade
Storage RT/4°C    Shipping RT
Components shown as mg/Liter
Inorganic Salts:
Ammonium Metavandate0.000585
Ammonium Molybdate•4H2O0.001236
Calcium ChlorideAbsent
Cupric Sulfate0.000160
Ferrous Sulfate•7H2O1.39
Magnesium Sulfate180.57
Manganese Sulfate0.000121
Nickel Chloride•6H2O0.000001189
Potassium Chloride186.40
Potassium Phosphate Monob68.05
Sodium Chloride7014.00
Sodium Metasilicate•9H2O0.1421
Sodium Selenite0.005188
Stannous Chloride•2H2O0.000001128
Zinc Sulfate•7H2O0.0897736
Amino Acids:
L-Alanine8.909
L-Arginine52.27
L-Asparagine132.10
L-Aspartic Acid13.31
L-Cysteine•HCl11.029
L-Glutamic Acid14.71
L-Glutamine292.20
Glycine7.507
L-Histidine15.52
L-Isoleucine13.12
L-Leucine39.36
L-Lysine•HCl36.54
L-Methionine4.476
L-Phenylalanine4.956
L-Proline5.755
L-Serine31.53
L-Threonine35.73
L-Tryptophan6.126
L-Tyrosine9.06
L-Valine35.16
Vitamins:
Biotin0.007329
Choline Chloride13.96
Vitamin B120.13550
Folinic Acid, Calcium0.005115
Myo-Inositol18.02
Nicotinamide6.105
D-Pantothenic Acid•Ca0.2383
Pyridoxine•HCl0.06168
Riboflavin0.11292
Thiamine•HCl0.3373
Other:
Adenine0.1351
Thymidine0.07266
D-Glucose1441.3
HEPES Buffer7149.00
Lipoic Acid0.002063
Phenol Red, SodiumAbsent
Putrescine•2HCl0.000161
Sodium Pyruvate110.00
Total16.96g/liter
Basal nutrient medium used for the growth of mammary epithelial cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).

Suggested Supplements: BPE 0.4%, Epidermal Growth Factor 10ng/ml, Insulin 5ug/ml, Hydrocortisone 0.5ug/ml, Isoproterenol 10uM, Transferrin 5ug/ml, Ethanolamine 0.1mM, Phosphoethanolamine 0.1mM, Amphotericin B 50ng/ml,
Gentamicin 50ug/ml


Directions per Liter: Dissolve 16.96g in 800-900ml of ddH2O stirring gently until completely solubilized. Do NOT heat. If required, add sodium bicarbonate. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.

Appearance: White to off-white, homogenous, free flowing powder

Solubility: Colorless to yellow, clear and complete. May form precipitate.

pH: As Reported

Endotoxin: 1EU/ml


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

US Biological application reference: Partanen, J.I. et al., (2007) PNAS 104:14694-14699. 1. Hammond, S. L. et al. Proc. Natl. Acad. Sci. USA Vol. 81; 5435-5439 (1984). 2. Peehl, D.M. and Ham, R.G., In Vitro: 16:526 (1980). 3. Bettger, W.J. et al., Proc. Natl, Acad. Soc., USA:78:, 5588-5592 (1981). 4. Stampfer, M.R. and Bartley, J. C. PNAS 82; 2394-2398 (1985)