Technical Data
Munc18 is a cytosolic brain protein of ~67kD which plays a key role in vesicle membrane fusion events with the plasma membrane. Functional interactions have been demonstrated to exist between Munc18, synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin, and syntaxin. Munc18 is the mammalian homologue of the C. elegans gene unc-18. Mutations in Munc18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect. Munc18 thus appears to be required by the synaptic vesicle fusion complex. Two Munc18 isoforms have recently been identified. Munc18-1 has been shown to be primarily expressed in brain, while Munc18-2 is expressed in most tissues.

Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Immunohistochemistry: Frozen sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceRabbit
Synthetic peptide corresponding to aa58-70, V58EDINKRREPIPS70 of human Munc18.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 1mg/ml BSA, 0.05% sodium azide.
Recognizes Munc18 at ~67kD by Western Blot. Species Crossreactivity: mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.