Technical Data
M2197-02
Munc18
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Technical Data |
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M2197-02 |
Munc18 |
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Description: Munc18 is a cytosolic brain protein of ~67kD which plays a key role in vesicle membrane fusion events with the plasma membrane. Functional interactions have been demonstrated to exist between Munc18, synaptosomal associated protein of 25kD (SNAP-25), synaptobrevin, and syntaxin. Munc18 is the mammalian homologue of the C. elegans gene unc-18. Mutations in Munc18 can result in accumulations of acetylcholine and paralytic phenotype suggestive of a neurotransmitter release defect. Munc18 thus appears to be required by the synaptic vesicle fusion complex. Two Munc18 isoforms have recently been identified. Munc18-1 has been shown to be primarily expressed in brain, while Munc18-2 is expressed in most tissues. Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilutions: Western Blot: 1ug/ml Immunohistochemistry: Frozen sections Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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