Technical Data
M2345X
MAP1B (MAP5, (Microtubule Associated Protein 1B) (BSA & Azide Free)
Description:
Microtubule-associated protein 1B (MAP1B, also known as MAP5, MAP1.2, MAP1(x), or MAP1X), is different from MAP1A (also known as MAP1 or MAP1.1). NEOMARKERS’ 3G5 MAb is highly specific to MAP1B and shows no cross-reaction with other MAPs, tau, and tubulin. Reportedly, MAP1B/MAP5 is an early MAP; it is present at high levels in embryonic and newborn rat brain and declines several-fold upon brain maturation. In several cellular situations, MAP1B/MAP5 is the first neuronal MAP to appear, and it is found in neurites from their very first emergence from the cell body. Expression of MAP1B/MAP5 is induced by nerve growth factor.

Applications:
Suitable for use in Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (formalin/paraffin): 1-2ug/ml for 30 minutes at RT. No special pretreatment is required for staining of formalin-fixed tissues.
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG10.N.393Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceBovineMouse
Concentration:
~1mg/ml
Immunogen:
Purified bovine brain MAPs. Cellular Localization: Cytoplasmic. MW of Antigen: 320kD Doublet
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See M2345.
Specificity:
Recognizes bovine microtubule associated protein 1B (MAP1B, also known as MAP5, MAP1.2, MAP1(x) or MAP1X). Does not crossreact with other MAPs, tau or tubulin. Species Crossreactivity: human and rat. Does not react with chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.