Technical Data
M2346-15E
MAP1LC3A (Microtubule-associated Proteins 1A/1B Light Chain 3A, Autophagy-related Protein LC3 A, Autophagy-related Ubiquitin-like Modifier LC3 A, MAP1 Light Chain 3-like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-associated Pro
Description:
MAP1A and MAP1B are microtubule-associated proteins made up of a heavy chain and multiple light chain components. One such light chain component associated with these proteins is APG8. APG8, an ~16kD mammalian homolog of yeast Atg8, is a cytoplasmic protein that plays an essential role in the process and regulation of autophagy. APG8 is an evolutionarily conserved protein that belongs to LC3 family. APG8 interacts with various proteins in the cytoplasm including mammalian homologues of Atg4, Atg7, Atg3 and Atg12 and gets modified into LC3-I and LC3-II respectively. Both of these forms are suggested to be involved in the formation and intracellular trafficking of autophagosomes in mammalian cells.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.25-1ug/ml
Immunohistochemistry (paraffin): 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Brain

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa1-50 of human APG8.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Specificity:
Recognizes human MAP1LC3A. Species Crossreactivity: bovine, opossum, rat, monkey. Species sequence homology: chimpanzee, mouse, pufferfish, xenopus, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kuono, T. et.al. J. Biol. Chem. 280:24610-24617 (2005). 2. Kabeya, Y. et.al. EMBO. J. 19:5720-5728 (2000).