Technical Data
M2346-17
MAP1LC3A, MAP1LC3B, ID (Microtubule-associated Proteins 1A/1B Light Chain 3A, Autophagy-related Protein LC3 A, Autophagy-related Ubiquitin-like Modifier LC3 A, MAP1 Light Chain 3-like Protein 1, MAP1A/MAP1B Light Chain 3 A, MAP1A/MAP1B LC3 A, Microtubule-
Description:
Microtubule-associated protein 1 light chain 3 (LC3) is a fibronectin mRNA-binding protein linked to mRNA translation. Three human isoforms of MAP1-LC3 have been cloned and characterized (MAP1- LC3A, -B, -C). These isoforms differ in their post-translation modification. MAP-LC3 proteins are related to cell growth, differentiation and migration in development and in diseases.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:5000-1:20,000
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Control Peptide: M2346-17P

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceZebrafishRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to the intermediate region of zebrafish MAP1-LC3.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in 40mM MOPS, pH 7.5, 0.1% BSA, 0.05% sodium azide, 50% glycerol.
Specificity:
Recognizes zebrafish MAP1-LC3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Zhou, B. et al. J. Clin. Invest. 100, 3070 (1997). 2. He, H. et al. J. Biol. Chem. 11, 11 (2003). 3. Jackson, W. et al. Plos Biol. 3, e156 (2005).