Technical Data
M2350-02
MAP2 (Microtubule Associated Protein 2)
Description:
MAP2 is a type II microtubule associated protein (MAP). It is located predominately in dendrites where it accelerates tubulin polymerization, cross-links microtubule fibers into stable bundles, and connects microtubule filaments to intermediate filaments. Alternative splicing of MAP2 mRNA gives rise to at least three different isoforms including; MAP2a (280kD), MAP2b (200kD), and MAP2c (70kD). The MAP2c isoform is expressed in embryos, whereas the MAP2a and MAP2b isoforms are expressed in adult tissues. MAP2 is the somatodentritic MAP of neurons, but it also is expressed to a lesser extent in glia.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-2ug/ml detects MAP2, ~300kD, in 20ug of rat brain microsomal protein preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with M2350-02 (0.5ug/ml). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry: 10ug/ml detects MAP2 in rat brain sections.
Optimal dilutions to be determined by the researcher.

Recommended secondary antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06F: IgG, H&L (FITC) (X-Adsorbed) Pab Gt xMo
I1904-23: IgG, X-Adsorbed (DTAF) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.T.88Affinity Purified
SizeStorageShippingSourceHost
200ug-20CBlue IceRatMouse
Concentration:
~0.5mg/ml
Immunogen:
Purified rat brain microtubules MAP2.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS pH 7.6, 0.1% sodium azide.
Specificity:
Recognizes rat MAP2 at ~300kD. Species Crossreactivity: human, rabbit and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Shelanski, et al., PNAS 70: 765768, (1973).