		Technical Data
M2352-01	MAP Kinase p38 alpha (MAPK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CSBP, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CASID-binding Protein, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1, MXI2)

Description:
p38 MAP kinase (MAPK) is also called RK (3) or CSBP (2). It is the mammalian homologue of the yeast HOG kinase. It participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP (kinase, alpha, beta, gamma and delta) have been identified. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

Applications:
Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000. Incubate membrane with diluted M2352-01 in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4°C with gentle shaking, overnight.
Flow Cytometry: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Type	Isotype	Clone	Grade
Mab	IgG2b	3A31	Purified

Size		Storage	Shipping	Source	Host
100ul		4°C (-20°C Glycerol)	Blue Ice	Human	Mouse

Concentration:

As reported

Immunogen:

MBP-p38 MAP kinase recombinant protein.

Purity:

Purified.

Form

Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.

Specificity:

Detects endogenous levels of p38 MAP kinase. Does not crossreact with either JNK/SAPK or p42/44 MAP kinase. Species Crossreactivity: Human, mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Han, J., et al., Science 265: 808-811 (1994). 2. Lee, J.C., et al., Nature 372: 739-746 (1994). 3. Rouse, J., et al., Cell 78: 1027-1037 (1994). 4. Freshney, N.W., et al., Cell 78: 1039:1049 (1994). 5. Raingeaud, J., et al., J. Biol. Chem. 270: 7420-7426 (1995). 6. Zervos, A.S., et al., PNAS. USA 92:, 10531-10534 (1995). 7. Zhao, M., et al., Mol. Cell. Biol. 19:, 21-30 (1999). 8. Yang, S.H., et al., Mol. Cell. Biol. 19: 4028-4038 (1999).