Technical Data
M2352-01
MAP Kinase p38 alpha (MAPK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CSBP, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CASID-binding Protein, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1, MXI2)
Description:
p38 MAP kinase (MAPK) is also called RK (3) or CSBP (2). It is the mammalian homologue of the yeast HOG kinase. It participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP (kinase, alpha, beta, gamma and delta) have been identified. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at threonine 180 and tyrosine 182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

Applications:
Suitable for use in ELISA, Western Blot and Flow Cytometry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:2000. Incubate membrane with diluted M2352-01 in 1X TBS, 5% nonfat dry milk, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Flow Cytometry: 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b3A31Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
As reported
Immunogen:
MBP-p38 MAP kinase recombinant protein.
Purity:
Purified.
Form
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 0.02% sodium azide, 50% glycerol.
Specificity:
Detects endogenous levels of p38 MAP kinase. Does not crossreact with either JNK/SAPK or p42/44 MAP kinase. Species Crossreactivity: Human, mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Han, J., et al., Science 265: 808-811 (1994). 2. Lee, J.C., et al., Nature 372: 739-746 (1994). 3. Rouse, J., et al., Cell 78: 1027-1037 (1994). 4. Freshney, N.W., et al., Cell 78: 1039:1049 (1994). 5. Raingeaud, J., et al., J. Biol. Chem. 270: 7420-7426 (1995). 6. Zervos, A.S., et al., PNAS. USA 92:, 10531-10534 (1995). 7. Zhao, M., et al., Mol. Cell. Biol. 19:, 21-30 (1999). 8. Yang, S.H., et al., Mol. Cell. Biol. 19: 4028-4038 (1999).