Technical Data
M2352-01A
MAP Kinase p38 alpha (MAPK14, Mitogen-activated Protein Kinase p38 alpha, Mitogen-activated Protein Kinase 14, CSBP, Cytokine Suppressive Anti-inflammatory Drug-binding Protein, CASID-binding Protein, SAPK2A, CSBP, CSBP1, CSBP2, CSPB1, MXI2)
Description:
P38 MAP kinase (MAPK), also called RK (3) or CSBP (2), is the mammalian homologue of the yeast HOG kinase and participates in a signaling cascade controlling cellular responses to cytokines and stress (1-4). Four isoforms of p38 MAP kinase, alpha, beta, gamma and delta, have been identified so far. Like the SAPK/JNK pathway, p38 MAP kinase is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light and growth factors (1-5). MKK3 and SEK activate p38 MAP kinase by phosphorylation at Thr180 and Tyr182. Activated p38 MAP kinase has been shown to phosphorylate and activate MAPKAP kinase 2 (3) and to phosphorylate the transcription factors ATF-2 (5), Max (6) and MEF2 (5-8).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:50. Requires antigen retrieval using citrate method. Antibody diluten: TBST/5% normal goat serum
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide (KLH coupled) corresponding to the C-terminal residues of human p38 MAP kinase alpha.
Purity:
Purified by protein A and peptide affinity chromatography.
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, and 50% glycerol.
Specificity:
Detects endogenous levels of total p38 MAP kinase alpha isoform independent of phosphorylation. Does not crossreact with other p38 MAP kinase isoforms such as beta, gamma or delta. Species Crossreactivity: Human, mouse, rat and monkey.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Han, J. et al. (1994) Science 265, 808811.(2) Lee, J.C. et al. (1994) Nature 372, 739746. (3) Rouse, J. et al. (1994) Cell 78, 10271037. 4) Freshney, N.W. et al. (1994) Cell 78, 10391049. (5) Raingeaud, J. et al. (1995) J. Biol. Chem. 270,74207426. (6) Zervos, A.S. et al. (1995) Proc. Natl. Acad. Sci.USA 92, 1053110534. (7) Zhao, M. et al. (1999) Mol. Cell. Biol. 19, 2130. (8) Yang, S.H. et al. (1999) Mol. Cell. Biol. 19, 40284038.