		Technical Data
M2352-03T	MAP Kinase 14, phosphorylated (Thr180, Tyr182) (Mitogen-activated Protein Kinase 14, MAPK 14, MAPK14, Mitogen-activated Protein Kinase p38alpha, MAP Kinase p38alpha, Cytokine Suppressive Anti-inflammatory Drug binding Protein, CSAID Binding Protein, CSBP,

Description:
MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.

Applications:
Suitable for use in ELISA and Dot Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Dot Blot: 1:500
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
200ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.25mg/ml

Immunogen:

Synthetic bisphosphopeptide incorporating amino acid residues surrounding phosphorylated Thr180 and Tyr182 of human MAPK14 (KLH).

Purity:

Purified by Protein A affinity chromatography.

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes human MAPK14 when phosphorylated at Thr180 and Tyr182.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Cheung, P.C., et al., EMBO J. 22(21):5793-5805 (2003). 2.Dean, J.L., et al., J. Biol. Chem. 278(41):39470-39476 (2003). 3.Sun, A., et al., Exp. Neurol. 183(2):394-405 (2003). 4.Yustein, J.T., et al., Oncogene 22(40):6129-6141 (2003). 5.Frevel, M.A., et al., Mol. Cell. Biol. 23(2):425-436 (2003).