Technical Data
MAP Kinase p44/42, phosphorylated (Thr202/Tyr204) (MAPK3/MAPK1, Mitogen-activated Protein Kinase 1, MAP Kinase 1, MAPK 1, ERT1, Extracellular Signal-regulated Kinase 2, ERK-2, MAP Kinase Isoform p42, p42-MAPK, Mitogen-activated Protein Kinase 2, MAP Kinas
Mitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines (1-3) and is an important target in the diagnosis and treatment of cancer (4). Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family as well as Mos and Tpl2/Cot. MEK1 and MEK2 are the primary MAPKKs in this pathway (5,6). MEK1 and MEK2 activate p44 and p42 through phosphorylation of activation loop residues Thr202/Tyr204 and Thr185/Tyr187, respectively. Several downstream targets of p44/42 have been identified, including p90RSK (7) and the transcription factor Elk-1 (8,9). p44/42 are negatively regulated by a family of dual-specificity (Thr/Tyr) MAPK phosphatases, known as DUSPs or MKPs (10), along with MEK inhibitors such as U0126 and PD98059.

Suitable for use in Western Blot, Immunofluorescence and Flow Cytometry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000. Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Immunofluorescence (IF-IC): 1:200
Flow Cytometry: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceHumanRabbit
Not Determined
Synthetic phosphopeptide corresponding to residues surrounding Thr202/Tyr204 of human p44 MAP kinase.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, <0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of human p44 and p42 MAP Kinase (Erk1 and Erk2) when dually phosphorylated at Thr202 and Tyr204 of Erk1 (Thr185 and Tyr187 of Erk2), and singly phosphorylated at Tyr204. Does not crossreact with corresponding phosphorylated residues of either JNK/SAPK or p38 MAP kinase. Species Crossreactivity: mouse, rat, monkey, mink, porcine, Drosophila, zebrafish
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Roux, P.P. and Blenis, J. (2004) Microbiol Mol Biol Rev 68, 320-44. 2. Baccarini, M. (2005) FEBS Lett 579, 3271-7. 3. Meloche, S. and Pouyssegur, J. (2007) Oncogene 26, 3227-39. 4. Roberts, P.J. and Der, C.J. (2007) Oncogene 26, 3291-310. 5. Rubinfeld, H. and Seger, R. (2005) Mol Biotechnol 31, 151-74. 6. Murphy, L.O. and Blenis, J. (2006) Trends Biochem Sci 31, 268-75. 7. Dalby, K.N. et al. (1998) J Biol Chem 273, 1496-505. 8. Marais, R. et al. (1993) Cell 73, 381-93. 9. Kortenjann, M. et al. (1994) Mol Cell Biol 14, 4815-24. 10. Owens, D.M. and Keyse, S.M. (2007) Oncogene 26, 3203-13.