Technical Data
M2355-01A
MAPKAP Kinase 2 (Mitogen-activated Protein Kinase-activated Protein Kinase 2, MAP Kinase-activated Protein Kinase 2, MAPK-activated Protein Kinase 2, MAPKAPK2, MAPKAPK-2, MK2)
Description:
MAPKAP kinase-2 (or MK2) is an acronym for mitogen-activated protein kinase-activated protein
kinase-2. This enzyme is phosphorylated and activated by Erks and p38 MAPK in vitro. While multiple residues of MK2 are phosphorylated in vivo in response to stress, only four of the
residues Thr25, Thr222, Ser272 and Thr334 are phosphorylated by p38 in an in vitro kinase assay. MK2 is serine-threonine protein kinase that contains a proline rich sequence and two
putative SH3-binding sites. MK2 phosphorylates downstream components on a consensus Pro-X-Ser/Thr-Pro motif. The bestcharacterized MK2 substrate is hsp27. Although unknown, the
physiological function of MK2 may be to increase stress resistance. MK2 is an essential component in the inflammatory response that regulates biosynthesis of TNF-a at a posttranscriptional level and MK2 is rapidly phosphorylated and activated in response to cytokines, stress and chemotactic factors.

See Control Peptide M2355-01A1

Applications:
Suitable for use in ELISA, Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
ELISA: 1:15000
Western Blot: 1:2000

Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%)aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa 310-325 of MAPKAP Kinase 2 (MTSALATMRVDYEQIK) conjugated to KLH using maleimide. A terminal cysteine residue was added to facilitate coupling.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.01% sodium azide.
Specificity:
Recognizes MAPKAP. Detects a single 44kD protein as indicated in crude extracts prepared from either unstimulated or TNF stimulated human HeLa cell lysates. This sequence is conserved 100% in human, mouse, rabbit, rat, hamster, chicken, Xenopus and zebrafish.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Kotlyarov A, et al.ms. Nat Cell Biol. Jun;1(2):94-7. 2. Cheng TJ , J Cell Biochem, 89(3): 589-602. 3. Rousseau S ,et al., EMBO J, 21(23): 6505-14.4. Verma A,et al., J Immunol,168:12, 5984-8.5. Neininger A,. J Biol Chem, 277:5, 3065-86. Rouse J, et al., Cell,78:6, 1027-37. 7. Zu YL Biochem Biophys Res Commun 200:2, 1118-24. 8. Knauf U,et al., EMBO J. 13:1, 54-60. 9. Engel K,et al.,FEBS Lett: 336:1, 143-7. 10. Stokoe D, et al., Biochem J:296 ( Pt 3), 843-849.