Technical Data
Marine Broth (Zobell)
Storage RT/4C    Shipping RT
Formulation per Liter g/Liter
Ammonium Nitrate0.0016
Boric Acid0.022
Calcium Chloride1.80
Ferric Citrate0.10
Magnesium Chloride, anhydrous5.90
Magnesium Sulfate3.24
Potassium Bromide0.08
Potassium Chloride0.55
Sodium Bicarbonate0.16
Sodium Chloride19.45
Sodium Fluoride0.0024
Sodium Phosphate Dibasic0.008
Sodium Silicate0.004
Strontium Chloride0.034
Yeast Extract1.00
Suitable for use in culturing heterotropic marine bacteria. This formulation simulates the mineral composition of sea water.

Directions per Liter: Suspend 37.35g in one liter of purified water. Mix thoroughly. Heat with stirring and boil for one minute to dissolve the powder completely. Aliquot into appropriate containers and autoclave at 121C at 15 psi for 15 minutes.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Beige, homogeneous, free flowing powder Solubility

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. J. Marine Research 4:42 (1941).