Technical Data
Marine Agar (Zobell)
Storage RT/4C    Shipping RT
Formulation per Liter g/Liter
Bacti Agar15.00
Ammonium Nitrate0.0016
Boric Acid0.022
Calcium Chloride1.80
Ferric Citrate0.10
Magnesium Chloride, anhydrous5.90
Potassium Bromide0.08
Potassium Chloride0.55
Sodium Bicarbonate0.16
Sodium Chloride19.45
Sodium Fluoride0.0024
Sodium Phosphate Dibasic0.008
Sodium Sulfate3.24
Sodium Silicate0.004
Strontium Chloride0.034
Yeast Extract1.00
Total: 52.352g/Liter
Suitable for use in culturing heterotropic marine bacteria. This formulation simulates the mineral composition of sea water.

Directions per Liter: Dissolve 52.35 grams in 800-900ml of ddH2O stirring gently with heating until completely solubilized. Adjust pH of the medium to the desired level. Add additional water to bring the solution to1L. If required, add sodium bicarbonate. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: Off-white to tan, homogeneous, free flowing powder

Solubility: Light yellow, clear, complete after autoclaving

pH: 6.8 0.4

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

J. Marine Research, 4:42 (1941)