Technical Data
Marine Broth w/o Sodium Phosphate (Zobell)
Storage RT/4C    Shipping RT
Formulation per Liter g/Liter
Ammonium Nitrate0.0016
Boric Acid0.022
Calcium Chloride1.80
Ferric Citrate0.10
Magnesium Chloride, anhydrous8.8
Potassium Bromide0.08
Potassium Chloride0.55
Sodium Bicarbonate0.16
Sodium Chloride19.45
Sodium Fluoride0.0024
Sodium Phosphate DibasicAbsent
Sodium Silicate0.004
Sodium Sulfate3.24
Strontium Chloride0.034
Yeast Extract1.00
Note: Add 0.008g/Liter of Sodium Phosphate Dibasic if needed.
Suitable for use in culturing heterotropic marine bacteria. This formulation simulates the mineral composition of sea water.

Directions per Liter: Suspend 40.244 grams in one liter of distilled/deionized (DDI) water. Heat with frequent stirring and boil for 1 minute to completely dissolve the powder. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Cool to 50C, mix well and dispense into plates. The prepared medium is slightly opalescent and may present a light precipitation. It is recommended to homogenize the medium in its container before pouring into plates.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White to beige, homogeneous, free flowing powder

Solubility: Light yellow, slightly turbid (will form a slight precipitate)

pH: As reported

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. J. Marine Research 4:42 (1941).