Technical Data
MARK1 (MAP/microtubule Affinity-regulating Kinase 1, MARK, KIAA1477, PAR1 Homolog c, Par1c, Par-1c, Serine/threonine-protein Kinase MARK1)
Microtubule associated proteins regulate the stability of microtubules, controlling processes such as cell polarity/differentiation, neurite outgrowth, cell division and organelle trafficking. The MARK (MAP/microtubule affinity-regulating kinases) family (MARK1-4) of serine/threonine kinases were identified based on their ability to phosphorylate microtubule associated proteins (MAPs) including tau, MAP2 and MAP4 (2-6). MARKs phosphorylate MAPs within their microtubule binding domains, causing dissociation of MAPs from microtubules and increased microtubule dynamics (2-4). In the case of tau, phosphorylation has been hypothesized to contribute to the formation of neurofibrillary tangles observed in Alzheimer's disease. Overexpression of MARK leads to hyperphosphorylation of MAPs, morphological changes and cell death. The tumor suppressor kinase LKB1 phosphorylates MARK and the closely related AMP-kinases within their T-loops, leading to increased activity.

Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (Formalin fixed paraffin embedded): 2.5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
50ug-20CBlue IceHumanRabbit
Synthetic peptide corresponding to internal domain of MARK1 (KLH).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human MARK1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.