Technical Data
M2410-13C
MART-1, multiple clones (Melanoma Antigen Recognized by T-cells 1, Melan-A)
Description:
Melanoma Antigen Recognized by T cells (MART-1), a widely shared melanoma epitope, is a 118aa protein with an approximate molecular weight of 18kD. MART-1 is recognized by HLA-A2-restricted autologous and allogeneic tumor-infiltrating lymphocytes from patients with established melanoma. MART-1 is expressed by a large proportion of melanomas and also in human and murine melanocyte lineage cells derived from skin, uvea, and retina. MART-1 is not
expressed in other normal tissues, including T cells, B cells, kidney epithelial cells, and fibroblasts. The antigen is not detectable in non-melanoma malignancies, including neoplasms of colon, breast, brain, kidney, lung and bone.

Applications:
Suitable for use in Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Frozen and formalin-fixed, paraffin embedded tissue sections. Requires HEIR with Citrate Buffer, pH 6.0
Optimal dilutions to be determined by the researcher.

Positive Control:
Melanoma

Expected Staining Pattern:
Cytoplasmic

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1+IgG2b+IgG2b6A118Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceMouse
Concentration:
~0.01mg/ml
Immunogen:
Human MART-1 fusion protein.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 1% BSA, 0.1% sodium azide.
Specificity:
Recognizes human MART-1 (Melan-A).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.