Technical Data
M2412-50D
Maspin (SERPINB5 , Serpin Peptidase inhibitor, Clade B (ovalbumin), member 5, Peptidase inhibitor 5, PI5, PI-5, Serpin B5)
Description:
Serpin B5, also known as Maspin, is a secreted member of the Serpin family although it has no known serine protease inhibitor activity. It is produced by mammary epithelial cells and functions as a tumor suppressor. Serpin B5 is a valuable biomarker of tumor progression and overall prognosis.

Applications:
Suitable for use in Direct ELISA, Western Blot, and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml. The detection limit for recombinant human Maspin is ~100ng/lane under non-reducing conditions and 25ng/lane under reducing conditions.
Immunoprecipitation: 25ug/ml. Conditioned cell culture medium spiked with recombinant human Maspin
Direct ELISA: 0.5-1ug/ml. The detection limit for recombinant human Maspin is ~15ng/well
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b9H158Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRat
Concentration:
~0.5mg/ml (after reconstitution)
Immunogen:
Recombinant protein corresponding to aa1-375 of human Maspin expressed in E. coli (Accession #NP_002630).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Specificity:
Recognizes human Maspin. Does not react with recombinant human Maspin A5, B6, B8 or B9. Species Sequence Homology: chimpanzee, 99%; canine, 92%; mouse, 89%; rat, 88%; chicken, 64%
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.