Description:
Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications not tested.
Recommended Dilution:
ELISA: 1:1000
Western Blot: 1:500
Immunohistochemistry (Frozen or paraffin): 1:200
Optimal dilutions to be determined by the researcher.
Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 6D552 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | Human | Mouse |
|
| Concentration: |
| ~1mg/ml (after reconstitution) |
| Immunogen: |
| Synthetic peptide corresponding to aa3-15 of non-phosphorylated human MGP (matrix Gla protein). Species Sequence Homology: rat: 60%, mouse: 29%, porcine: 15% and equine: 19%. |
| Purity: |
| Purified by Protein G affinity chromatography. |
| Form |
| Supplied as a lyophilized powder from PBS, 0.05% sodium azide. Reconstitute with 100ul sterile ddH2O. |
| Specificity: |
| Recognizes human carboxylated (functional) and non-carboxylated (non-functional) MGP. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Assay for Human Matrix Gla Protein in Serum: Potential Applications in the Cardiovascular Field: L.A. Braam, et al.; Arterioscler. Thromb. Vasc. Biol. 20, 1257 (2000) 2. Role of Vitamin K and vitamin K-dependent proteins in vascular calcification: L.J. Schurgers, et al.; Z. Kardiol. 90, 57 (2001) 3. Matrix gla protein accumulates at the border of regions of calcification and normal tissue in the media of the arterial vessel wall: H.M. Spronk, et al.; BBRC 289, 485 (2001)
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