Technical Data
M2420-13
Matrix Metalloproteinase 1 (MMP-1, Fibroblast/Interstital Collagenase-1)
Description:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes a secreted enzyme which breaks down the interstitial collagens, types I, II, and III. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3.

Applications:
Suitable for use in Immunohistochemistry, Western Blot and ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: PLP fixed paraffin-embedded tissues required for best results.
Western Blot: 1ug/ml
Optimal dilution determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG2a,k2Q519Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~2mg/ml
Immunogen:
Synthetic peptide containing aa332-350 of human MMP-1.
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.0, 2% BSA (protease free).
Specificity:
Recognizes the precursor and active forms of human MMP-1. Does not react with human MMP-2, 3, 8, 9, 13 and bovine MMP-1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Okada, Y. et al. (1990). Virchows Archiv. B Cell Pathol. 59: 305312. 2. Zhang, J. et al. (1993). Clin. Chim. Acta. 219: 114. 3. Matsuki, H. et al. (1996). Clin. Chim. Acta. 244: 129143. 4. Knauper, V. et al. (1996). J. Biol. Chem. 271: 15441550.