Technical Data
M2421-19
Matrix Metalloproteinase 3 (MMP-3, Stromelysin-1, SL-1, Transin)
Description:
MMPs are frequently expressed in malignant neoplastic cells and are thought to play crucial roles in tumor invasion and metastasis. Tissue inhibitors of matrix metalloproteinases (TIMPs) represent a family of ubiquitous proteins whose main biological function resides in their ability to control the activity of MMPs. MMP secretion by microvascular endothelial cells is an essential initial step in the formation of new blood vessels (neo-angiogenesis). Since angiogenesis does not occur in large blood vessels, it was investigated whether the secretion of MMPs and TIMPs differs between micro- and macro-vascular endothelial cells. Cultured endothelial cells derived from microvascular vs. macrovascular tissues exhibit different MMP and TIMP secretory profiles.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin-embedded tissues
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2Q522Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRabbitMouse
Concentration:
~2mg/ml
Immunogen:
Rabbit uterine cervical fibroblasts.
Purity:
Purified
Form
Supplied as a liquid in 0.1M PBS, pH 7.0, 2% BSA (protease free).
Specificity:
Recognizes the precursor and active forms of rabbit MMP-3. Does not crossreact with mouse or rat MMP-3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Ito, A., et al., Comp. Biochem. Physiol. 99B: 381-385 (1991). 2. Morita, Y., et al., Connect. Tissue 27: 183-190 (1995).