Technical Data
M2425-02A
Matrix Metalloproteinase 9 (MMP-9, MMP9, 92kD Gelatinase, 92kD Type IV Collagenase, Collagenase Type 4 beta, CLG4B, Collagenase Type IV 92kD, Collagenase 4, EC 3.4.24.35, Gelatinase 92kD, Gelatinase B, Gelatinase beta, GELB, Macrophage Gelatinase, Type V
Description:
MMPs are a group of enzymes involved in matrix degradation. They share some important characteristics, such as a common mode of activation, a conserved amino acid sequence in the putative metal binding-active site region, and specific proteinase inhibitors known as tissue inhibitors of metalloproteinases (TIMPs). Reportedly, MMPs play a crucial role in tumor cell invasion and metastasis.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Immunoprecipitation: 2ug/mg
Western Blot: 1-2ug/ml. Under non-reducing conditins only.
Immunohistochemistry: Frozen
Optimal dilutions to be determined by the researcher.

Positive Controls:
Conditioned, serum-free medium from (TPA-treated) human fibrosarcoma HT-1080 cells or CCL4 cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k8L694Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Full length human native 92kDa MMP-9 protein. Cellular Localization: Secreted
Purity:
Purified
Form
Supplied as a liquid in PBS, pH 7.4, 0.2%BSA, 0.09% sodium azide
Specificity:
Recognizes pro (latent) and active forms of HUMAN MMP-9. Also reacts with the complex (~200kDa) of MMP-9 and tissue inhibitor of metalloproteinases-1 (TIMP-1). By Western Blot, a band at 92kDa (pro form) and ~86kDa (active form) which corresponds to the predicted molecular weight of MMP-9 is seen. No crossreaction with pro and active forms of MMP-2. Species Crossreactivity: rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Nikkari ST, et. al. American Journal of Pathology, 1996, 149 (5):1427-33. 2. Soini Y, et. al. Journal of Histochemistry and Cytochemistry, 1994, 42(7):945-51. 3. Hurskainen T, et. al. Journal of Histochemistry and Cytochemistry, 1996, 44(12):1379-88. 4. Schnaper HW, et. al. Journal of Cellular Physiology, 1993, 156(2):235-46. 5. Visscher DW, et. al. International Journal of Cancer, 1994, 59(3):339-44.