Technical Data
M2425-21
Matrix Metalloproteinase 9 (MMP-9, Collagenase IV, Gelatinase B)
Description:
All cells within tissues are surrounded by an extracellular matrix (ECM) giving the tissues shape and structure. The ECM is constantly being remodeled and constant communication is maintained between cells through this matrix. Secreted proteins, termed matrix metalloproteinases (MMPs), are involved in the modulation of cell matrix interactions. MMPs are Zn(2+) binding endopeptidases that degrade various components of the ECM. MMPs are enzymes implicated in normal and pathologic tissue remodeling processes, wound healing, angiogenesis, and tumor invasion. These enzymes are very potent when active, and are associated with extracellular space inhibitors called TIMPs (tissue inhibitors of matrix metalloproteinases). MMP9, also known as gelatinase B, is a secreted enzyme which degrades the interstitial collagens, types I, II, and III and is produced by normal alveolar macrophages and granulocytes.

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:500. Detected MMP-9 on 10ug of human spleen lysates. Human spleen lysates were resolved by electrophoresis, transferred to PVDF membrane and probed with M2425-21 (1:500). Proteins were visualized using an IgG (HRP) goat anti-mouse and a chemiluminescence detection system.
Immunohistochemistry (Frozen, paraffin): 1-5ug/ml. PLP Fixation preferred. Will also work on formalin fixed tissues.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k2Q521Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa626-644 of human MMP-9, RSASEVDRMFPGVPLDTHD. Epitope: Near C-terminus.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 0.1M phosphate buffer, pH 7.0, 2% BSA (protease free).
Specificity:
Recognizes human MMP-9. Recognizes precursor (92kD) and active (83kD) forms of human and guinea pig MMP-9. Does not react with the human MMP-1, 2, 3 or 13. Species Crossreactivity: guinea pig, rat and rabbit. Does not react with mouse, hamster, or bovine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Attiga, F., et al., Cancer Research 60: 4629-4637 (2000). 2. Iizasa, T., et al., Clinical Can. Res. 5: 149-153 (1999). 3. Romanic, A., et al., Stroke 29: 1020-1030 (1998). 4. Nomura, H., et al., Int. J. Cancer 69: 9-16 (1996). 5. Nakagawa, T., et al., J. Neurosurg. 81: 69-77 (1994). 6. Kawahara, E., et al., Jpn. J. Cancer Res. 84: 409-418 (1993). 7. Fujimoto, N., et al., Clin. Chim. Acta. 221: 91-103 (1993).