Technical Data
M2472-01A
MBD2a (Methyl CpG Binding Domain Protein 2a, Demethylase, DMTase, DKFZp586O0821, MBD2, Methyl CpG Binding Domain Protein 2 Isoform 1, Methyl CpG Binding Protein MBD2, NY-CO-41)
Description:
The MBD family of proteins includes MeCP2, MBD1, MBD2, MBD3 and MBD4. MBD2 is highly similar to MBD3 but only MBD2 targets the MeCP1 complex to methylate DNA. MBD2 has two isoforms, MBD2a and MBD2b. MBD2a is a 414aa protein, whereas MBD2b lacks the 152aa N-terminal extension. MBD2a interacts with RNA helicase A to enhance CREB-dependent gene exptression.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-4ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa164 from rat MBD2a.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.5% BSA, 5mM EDTA, 0.01% thimerosal, 30% glycerol.
Specificity:
Recognizes rat MBD2a. Species Crossreactivity: human and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.