Technical Data
M2690-01A
MCAF, Recombinant, Human (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)
5ug
20ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant human MCP-1 is a non-glycosylated, polypeptide chain containing 76 amino acids.

Endotoxin:
< 0.01ng/ug or < 0.1EU/ug as per LAL

Biological Activity:
MCP-1 is fully biologically active when compared to standard. The specific activity is determined by the ability of MCP-1 to chemoattract human Monocytes using a concentration of 5-20ng/ml.

AA Sequence:
QPDAINAPVT CCYNFTNRKI SVQRLASYRR ITSSKCPKEA VIFKTIVAKE ICADPKQKWV QDSMDHLDKQ TQTPKT

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O to concentration of 0.1-1mg/ml with carrier protein (1% HSA or BSA). Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
8.7kD
Source: Recombinant, human, produced in E. coli
Purity: 98% as determined by RP-HPLC; Reducing and non-reducing SDS-PAGE.
Form: Supplied as a lyophilized powder. Reconstitute with sterile ddH2O to 0.1-1mg/ml, which can be further diluted into other aqueous solutions. For long term storage, it is recommended to add a carrier protein (0.1% HSA or BSA).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.