Technical Data
MCAF, Recombinant, Human (MCP-1, Macrophage/Monocyte Chemotactic and Activating Factor)
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant corresponding to human MCAF expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50=3.0-10ng/ml. The biological activity was determined by measuring dose dependent chemotaxis with human THP-1 cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile dH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Purity: ~98% (SDS-PAGE)
Concentration: Not determined
Form: Supplied as a lyophilized powder. BSA free. Reconstitute with sterile dH2O to 0.1-0.5mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Yoshimura, T., JE. FEBS Lett 244:487-93. 2. Cross, A.K.,et al., . Cytokine 9:521-8. 3. Rollins, B.J., A. Walz, and M. Baggiolini (1991). Blood 78:1112-6. 4. Rodriguez-Frade, J.M.,et al. Proc. Natl. Acad. Sci. USA 96:3628-33. 5. Cambien, B., et al.,. Blood 97:359-66.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.