Technical Data
Media
McBride Agar w/Cycloheximide (Powder)
M2695
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/liter
Agar15
Glycine Anhydride 10
Tryptose10
Sodium Chloride5
Beef Extract 3
Phenylethanol2.5
Supplied separately:
Cycloheximide 0.2
Total: 46.2g/Liter
Improved selectivity for isolation of Listeria monocytogenes.


Preparation of Cycloheximide Solution: Add 0.2g cycloheximide to ddH2O and bring volume to 10ml. Mix thoroughly. Filter sterilize.

Preparation of Medium: Weigh 46g of powdered medium and bring volume to 990ml. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 mins. at 121C and 15 psi pressure. Cool to 45-50C. Aseptically add 10ml sterile cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: As reported

Solubiliity: As reported

pH: 7.30.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.