Technical Data
Media
McBride Agar w/Cycloheximide (Powder)
M2695
Microbiological Grade
Storage RT    Shipping RT
Components shown as g/liter
Agar15
Glycine Anhydride 10
Tryptose10
Sodium Chloride5
Beef Extract 3
Phenylethanol2.5
Supplied separately:
Cycloheximide 0.2
Total: 46.2g/Liter
Improved selectivity for isolation of Listeria monocytogenes.


Preparation of Cycloheximide Solution: Add 0.2g cycloheximide to ddH2O and bring volume to 10ml. Mix thoroughly. Filter sterilize.

Preparation of Medium: Weigh 46g of powdered medium and bring volume to 990ml. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 mins. at 121°C and 15 psi pressure. Cool to 45-50°C. Aseptically add 10ml sterile cycloheximide solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.Storage and Stability:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept refrigerated and used within a short period of time.

Appearance: As reported

Solubiliity: As reported

pH: 7.3±0.2

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.